Sensitive Method to Identify and Characterize Proteinases In Situ after SDS-PAGE

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Sensitive method to identify and characterize proteinases in situ after SDS-PAGE.

Cells and body fluids contain numerous, different proteinases; to identify and characterize them are both important and difficult tasks. Especially difficult to identify and characterize are highly specific proteinases. Here, we present an extremely sensitive and quantitative method to characterize proteinases fractionated by SDS-PAGE that cleave specific rhodamine-based fluorogenic substrates....

متن کامل

Tricine – SDS - PAGE Hermann Schägger

Tricine–SDS-PAGE is commonly used to separate proteins in the mass range 1–100 kDa. It is the preferred electrophoretic system for the resolution of proteins smaller than 30 kDa. The concentrations of acrylamide used in the gels are lower than in other electrophoretic systems. These lower concentrations facilitate electroblotting, which is particularly crucial for hydrophobic proteins. Tricine–...

متن کامل

Abnormal SDS-PAGE migration of cytosolic proteins can identify domains and mechanisms that control surfactant binding.

The amino acid substitution or post-translational modification of a cytosolic protein can cause unpredictable changes to its electrophoretic mobility during SDS-PAGE. This type of "gel shifting" has perplexed biochemists and biologists for decades. We identify a mechanism for "gel shifting" that predominates among a set of ALS (amyotrophic lateral sclerosis) mutant hSOD1 (superoxide dismutase) ...

متن کامل

Capillary transfer as an efficient method of transferring proteins from SDS-PAGE gels to membranes.

1.Life Technologies. 1997. GIBCO BRL Products and Reference Guide, p. 14-1. Gaithersburg, MD. 2.Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed., p. 6.31 and 9.32. CSH Laboratory Press, Cold Spring Harbor, NY. 3.Smith, S.S., T.E. Gilroy and F.A. Ferrari. 1983. The influence of agarose-DNA affinity on the electrophoretic separation of DNA fragment...

متن کامل

comparative study of protein profile in mycobacterium tuberculosis and mycobacterium bovis with sds-page method

background: mycobacterium bovine, as the etiology of bovine tb, involves human in some cases and with mycobacterium tuberculosis are regarded as global health problems. the aim of this study was to extract and compare protein profile of these strains in order to achieve effective biomarkers for diagnosis and the vaccine components. materials and methods: first, the clinical samples were culture...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: BioTechniques

سال: 2000

ISSN: 0736-6205,1940-9818

DOI: 10.2144/00295rr07